Journal: PLOS ONE

Article Title: Clinical outcomes and anti-inflammatory mechanisms predict maximum heart rate improvement after physical activity training in individuals with psychiatric disorders and comorbid obesity

doi: 10.1371/journal.pone.0313759

Figure Lengend Snippet: MHR: maximum heart rate, HRSD: Hamilton rating scale for depression, PANSS-P: positive and negative syndrome scale (positive subscale), PANSS-G: positive and negative syndrome scale (general subscale), IL-10: interleukin 10, TNF-α: tumor necrosis factor alpha, ROS: reactive oxygen species; ΔΨm: mitochondrial membrane potential, VCAM: vascular cellular adhesion molecule, LEPMN: leukocyte-endothelium adhesion polymorphonuclear, RVPMN: rolling velocity polymorphonuclear cells, PSEL: P-selectin; MPO: myeloperoxidase.

Article Snippet: In total, 100 000 cells were plated in each well of 96-well plates and incubated for 30 min at 37°C with the corresponding fluorochromes, as follows: dichlorofluorescein diacetate to measure reactive oxygen species (ROS) production (485 nm excitation, 535 nm emission), MitoSOX to measure mitochondrial ROS (mROS) (510 nm excitation, 580 nm emission), tetramethylrodamin methyl ester to assess mitochondrial membrane potential (ΔΨm) (552 nm excitation, 574 nm emission), superoxide dismutase (SOD) (total SOD/T-SOD Activity Assay Kit–Colorimetric—from NOVUS), and 5-chloromethylfluorescein diacetate to measure intracellular glutathione (GSH) (492 nm excitation, 517 nm emission).

Techniques: Membrane

Journal: Journal of Cellular and Molecular Medicine

Article Title: The outer membrane protein Tp92 of Treponema pallidum induces human mononuclear cell death and IL ‐8 secretion

doi: 10.1111/jcmm.13879

Figure Lengend Snippet: Effect of Tp92 on the ROS levels in THP ‐1 cells. The cells were treated with Rosup (1 μg/mL) or Tp92 (5 μg/mL) for 12 hours. Average fluorescence intensity was measured by flow cytometry

Article Snippet: After harvesting, the cells were subjected to flow cytometry according to the protocol for the reactive oxygen species (ROS) assay kit (BD Biosciences) to determine ROS levels.

Techniques: Fluorescence, Flow Cytometry